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KMID : 1123920170310040220
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Korean Journal of Oriental Physiology and Pathology
2017 Volume.31 No. 4 p.220 ~ p.225
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Investigation of the Effect of Water Extract of Lithospermi Radix on the Expression of I L-1β, TNF-α and iNOS Genes in Raw 264.7 Cells
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Cho Nam-Joon
Choi Young-Ho
Lee Woong-Hee
Kim Kee-Kwang
Han Hyo-Sang
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Abstract
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Lithospermi Radix (LR) is known to have an anti-inflammatory effect. However, the mechanisms are not well known. In this study, LPS-induced mouse RAW 264.7 macrophage cells were treated with LR to investigate the time-dependent inflammation response of LR. RAW 264.7 cells were treated with various concentrations of LR for 24 hours, followed by MTS assay. Cell viability was increased at all experimental concentrations. The mRNA expression levels of IL-1β, TNF-α and iNOS were increased by treatment of RAW 264.7 cells with LR at a concentration of 200 μ g/ml for 6 hours and 24 hours. Treatment of LR with 200 μg/ml concentration for 6 hours promoted mRNA expression levels of IL-1β, TNF-α and iNOS in LPS-induced RAW 264.7 cells. However, IL-1β, TNF-α and iNOS mRNA expression was suppressed by treatment of LR with 200 μg/ml concentration for 24 hours in LPS-induced RAW 264.7 cells. These results suggest that the effect on inflammation of LR is promptly promoted and then to rapidly alleviate the inflammatory reaction. This study proposes that the time-dependent activities of herbal medicine is a very important factor in analyzing the anti-inflammatory effect of various herbal medicines including LR.
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KEYWORD
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Lithospermi Radix, MTS Assay, Anti-inflammation, RAW 264.7 cell, Lipopolysaccharide (LPS)
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